Workers at a Whole Foods Market in Philadelphia voted on Monday to become the first unionized store in Amazon’s grocery chain, opening a new front in the e-commerce giant’s efforts to fend off labor organizing in multiple segments of its business.

Employees at the sprawling Whole Foods store, in the city’s Spring Garden neighborhood, voted 130 to 100 in favor of organizing with the United Food and Commercial Workers union, the National Labor Relations Board said.

Store employees said they hoped a union could help negotiate higher wages, above the current starting rate of $16 an hour, and better benefits. Some longtime employees, who have been with Whole Foods since well before Amazon bought the chain in 2017, said reductions in benefits and cuts in staffing levels when Amazon took over, among other changes, had been sources of frustration.

But those leading the union campaign hinted at a broader goal: to inspire a wave of organizing across the chain’s more than 500 grocery stores, adding to union drives among warehouse workers and delivery drivers that Amazon is already combating.

“I expect others to follow, and that will increase the leverage that we have at the bargaining table,” said Ben Lovett, an employee at the Philadelphia store who has led the organizing. “We’ve shown them that it’s possible to organize at Amazon.”

“This fight is far from over,” Wendell Young IV, president of U.F.C.W. Local 1776, which represents food and retail workers in Pennsylvania, said in a statement, “but today’s victory is an important step forward.”